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Potato Dextrose Agar Media

Formula / Liter
Potato Infusion from (200 g) -4 g/ Dextrose-20 g/ Agar -15 g (4.0 g of potato extract is equivalent to 200 g of infusion from potatoes.)

Potato Dextrose Agar is used for the cultivation of fungi.

Preparation
Add Potato to water and simmer for 30-60 minutes, ,Filter through several layers of cheesecloth or muslin. ,Suspend 39 g of the medium in one liter of purified water. ,Heat with frequent agitation and boil for one minute to completely dissolve the medium. ,Autoclave at 121°C for 15 minutes in 15 psi (pounds per square inch)

Final pH: 5.6 ± 0.2 at 25°C


Corn Meal Agar Media

Corn Meal Agar is used for the cultivation of pathogenic fungi.

Formula/Liter
Corn Meal Infusion from (20g)2.0 g/Agar 15.0g/Dextrose 20g/Peptone 20g (2.0 g of Corn extract is equivalent to 20 g of infusion from Corn.)

Preparation
Add Corn to water and simmer for 30-60 minutes., Filter through several layers of cheesecloth or muslin., Suspend 57 g of the medium in one liter of purified water., Autoclave at 121°C for 15 minutes in 15 psi (pounds per square inch)

Final pH: 6.0 ± 0.2 at 25°C

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